The Streets of Killarney 5 Mile follows an entirely paved route that starts at Killarney House and Gardens and finishes on Beech Road in the town center. The first two miles navigate the scenic paths of the Killarney National Park (Demesne and Knockreer), passing landmarks like the Deenagh River and the Golden Gates. The final three miles consist of two laps through the town's central streets, including New Street and High Street, where local crowds typically gather to support runners. The route is described as fast and flat, providing a consistent tarmac surface throughout the circuit.